Journal: AIDS Research and Therapy

Article Title: Utility of the point of care CD4 analyzer, PIMA, to enumerate CD4 counts in the field settings in India

doi: 10.1186/1742-6405-9-26

Figure Lengend Snippet: Comparison of the CD4 counts obtained from finger prick sample and from paired venous blood . Figure A and C: Linear regression analysis: The correlation between the CD4 counts obtained from finger prick sample using PIMA CD4 analyzer (Y axis) and from venous blood using FACSCalibur (2A) and PIMA (2C) (X axis) is assessed. Figure B and D: The agreement analysis using the Bland Altman plots. The percent relative bias for CD4 counts from finger prick sample and the paired venous blood using FACSCalibur (Figure B) and PIMA (Figure D) is plotted on the Y axis and the average of the CD4 count by both the samples is plotted on X axis. The black line represents the mean% relative bias of where as the blue lines represent the range of Mean ± SD.

Article Snippet: Of these 21 centres, 2 had FACSCalibur, 13 had FACSCount (both from Becton Dickinson, USA) and 6 had CyFlow® SL3 counter from Partec, Germany.

Techniques: Comparison

Journal: Clinical and Experimental Immunology

Article Title: Modulation of the immune response to Mycobacterium tuberculosis during malaria/ M. tuberculosis co‐infection

doi: 10.1111/cei.12861

Figure Lengend Snippet: CD4+ T cell counts from patient groups. Patients were grouped according to disease diagnosis plus or minus tuberculosis (TB) chemotherapy into the following groups: controls (Cont), latent TB (Lat‐TB), active TB without chemotherapy (TB+drug–) or with chemotherapy (TB+drug+), TB‐HIV co‐infection (TB+HIV+) and TB‐malaria (TB+MP+) co‐infection. CD4 counts were determined using blood collected in ethylenediamine tetraacetic acid (EDTA) analysed with an automated Cyflow SL.3 cell cytometer. Counts are expressed as cells/μl. Graph shows median and 5–95 percentiles. Statistical significance between groups was determined using a Kruskal–Wallis test with Dunn's multiple comparison correction; *P < 0·05; **P < 0·01; ***P < 0·001; ****P < 0·0001. Significant differences among controls and latent TB and active TB groups are shown in grey. Significant differences among active TB groups are shown in black.

Article Snippet: Blood samples were collected in ethylenediamine tetraacetic acid (EDTA) vacutainer blood collection tubes (BD Biosciences, San Jose, CA, USA) and CD4 + T cell counts were measured within 5 h of sample collection using a Cyflow SL.3 cytometer for automated CD4 + counts, as per the manufacturer's instructions (Partec, Nuremberg, Germany).

Techniques: Infection, Cytometry